Untargeted metabolomics approach to discriminate mistletoe commercial products
نویسندگان
چکیده
Abstract Mistletoe ( Viscum album L.) is used in German-speaking European countries the field of integrative oncology linking conventional and complementary medicine therapies to improve quality life. Various companies sell extracts, fermented or not, for injection by subcutaneous intra-tumoral route with a regulatory status anthroposophic medicinal products (European Medicinal Agency (EMA) assessment status). These as well anthroposophical physicians argue that complex matrices composed many molecules mixture are necessary activity host tree mistletoe parasitic plant main determining factor this matrix composition. The critical point parenteral devices extracts do not have standard chemical composition regulated EMA guidelines, because they drugs, speaking. However, mechanism mistletoe’s anticancer its effectiveness treating supporting cancer patients fully understood. Because lack transparency knowledge regarding composition, we undertook an untargeted metabolomics study several explore compare their fingerprints LC-(HR)MS(/MS) 1 H-NMR. Unexpectedly, showed was primarily driven manufacturer/preparation method rather than different trees. This differential may cause differences immunostimulating anti-cancer activities commercially available illustrated structure–activity relationships based on LC–MS/MS H-NMR identifications completed docking experiments. In conclusion, order move towards evidence-based use mistletoe, it priority bring rigor quality, chemically
منابع مشابه
Untargeted metabolomics.
Along with genes and proteins, metabolites play important roles in sustaining life. Their functions include "primary" functions in metabolism and energy storage, as well as "secondary" functions in cell-to-cell signaling, metal acquisition, and virulence. There remains much to be learned about the in vivo roles of metabolites. Approaches that accelerate measurement of metabolite levels directly...
متن کاملUntargeted metabolomics: an emerging approach to determine the composition of herbal products
Natural remedies, such as those based on traditional Chinese medicines, have become more popular also in western countries over the last 10 years. The composition of these herbal products is largely unknown and difficult to determine. Moreover, since plants respond to their environment changing the metabolome, the composition of plant material can vary depending on the plant growth conditions. ...
متن کاملDissecting Bottromycin Biosynthesis Using Comparative Untargeted Metabolomics
Bottromycin A2 is a structurally unique ribosomally synthesized and post-translationally modified peptide (RiPP) that possesses potent antibacterial activity towards multidrug-resistant bacteria. The structural novelty of bottromycin stems from its unprecedented macrocyclic amidine and rare β-methylated amino acid residues. The N-terminus of a precursor peptide (BtmD) is converted into bottromy...
متن کاملUntargeted metabolomics suffers from incomplete data analysis
Introduction: Untargeted metabolomics is a powerful tool for biological discoveries. Significant advances in computational approaches to analyzing the complex raw data have been made, yet it is not clear how exhaustive and reliable are the data analysis results. Objectives: Assessment of the quality of data analysis results in untargeted metabolomics. Methods: Five published untargeted metabolo...
متن کاملUntargeted Metabolomics To Ascertain Antibiotic Modes of Action.
Deciphering the mode of action (MOA) of new antibiotics discovered through phenotypic screening is of increasing importance. Metabolomics offers a potentially rapid and cost-effective means of identifying modes of action of drugs whose effects are mediated through changes in metabolism. Metabolomics techniques also collect data on off-target effects and drug modifications. Here, we present data...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2021
ISSN: ['2045-2322']
DOI: https://doi.org/10.1038/s41598-021-93255-z